Ohio University’s Chemistry and Biochemistry Colloquium Series presents Dr. Abraham Badu-Tawiah on “Mass Spectrometry for Paper-based Immunoassays:
Towards On-demand Diagnosis” on Monday, Nov. 2, at 4:10 p.m. in Clippinger 194.
Dr. Abraham Badu-Tawiah
Badu-Tawiah is an Assistant Professor of Analytical Chemistry at The Ohio State University.
Abstract: The increasing needs of disease management have created new standards for diagnostic techniques to include patient-friendliness, sensitive and reliability. However, current analytical methods, either point-of-care testing or centralized detection are not able to meet all these criteria. In this presentation, I will describe the development of a two-point separation on-demand diagnostic strategy that could combine the advantages of convenient onsite sample operation with reliable centralized detection. This new methodology is based on a paper-based mass spectrometry immunoassay platform that adopts stable and cleavable ionic probes as mass reporter; the ionic probes make possible sensitive, interruptible, storable and restorable on-demand detection. This concept is successfully demonstrated via (i) the capture and detection Plasmodium falciparum histidine-rich protein 2, (ii) diagnosis of onset of liver injury by monitoring the conversion of L-alanine to pyruvate by alanine transaminase enzyme, and (iii) multiplexed and simultaneous detection of cancer antigen 125 and carcinoembryonic antigen from serum samples. The cleavable-charge-tag-based immunoassay strategy also represents a paradigm shift in which portable mass spectrometers can now be used for indirect analysis of large (kDa) biomolecules.
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